Scientists use zebrafish to understand the connection between the immune system and regeneration

How the immune system responds to injury in many organs and tissues allows and enables their repair and regeneration. Yet for some species like humans, damage to organs such as the brain, spinal cord, or heart is irreversible. Imagine if we were able to regenerate these. For organ transplant candidates and recipients, the nerve-wracking wait for “the call,” or the lifelong need for immunosuppressing medications would no longer be necessary.

New research from the Stowers Institute for Medical Research used the highly regenerative zebrafish to investigate the timing and genetic programs of macrophages, a type of white blood cell, in the repair and regeneration of a zebrafish sensory organ. Understanding how the immune system responds to injury, first by inducing inflammation immediately followed by an anti-inflammatory response, provides invaluable knowledge for designing targeted immunotherapies that may be applicable in combating human conditions like hearing loss or deafness, heart or spinal cord damage.

Recently published in Nature Communications on September 20, 2022, Postdoctoral Researcher Nicolas Denans, PhD, in the lab of Stowers Investigator Tatjana Piotrowski, PhD, discovered a new macrophage anti-inflammatory paradigm. Rather than the established view that anti-inflammatory activation states for macrophages are linked to just one type of signaling pathway, Denans found that the same population can, and must, transition through each of three anti-inflammatory states for organ regeneration.

“Organ regeneration offers an exciting opportunity for studying the immune system and to inquire why some species can regenerate organs like the heart or missing limbs while others like humans cannot,” said Piotrowski.

Zebrafish sensory organ hair cells are an ideal system to investigate the pathways and cell types involved in regeneration since they are easily destroyed with antibiotics and begin regenerating within five hours. This enabled the researchers to identify the exact timing and genetic programs for each anti-inflammatory macrophage activation state.

“Our hypothesis is that human macrophages do not receive the proper chemical activation “cocktail” to instruct pro-regenerative processes,” said Denans. “Identifying the molecular recipe of macrophage activation in zebrafish may one day enable us to design regenerative immunotherapies in humans.”

Essential for organ regeneration, macrophages, which in Latin literally translates as “big eaters,” engulf foreign particles like dead cells and bacteria and use enzymes to digest them. In addition to their culinary appetite, these cells signal both pro- and anti-inflammatory pathways to secrete chemicals, or cytokines to either recruit additional types of white blood cells or trigger anti-inflammatory pathways for cellular and tissue repair.

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